This study was under taken to monitor the production of protease enzyme from paddy field soil fungal isolates obtained from Mannargudi, Tamil Nadu. The study also sought to optimize the kinetic parameters of the enzyme with the aim of establishing large scale production of it. Two isolates Aspergillus flavus and Aspergillus niger out of the six were opted as potential candidates for enzyme production following the screening method of plate assay. The results observed were for the maximal production of enzyme by the isolates at various temperatures and pH. Aspergillus flavus and Aspergillus niger showed highest activity in the optimized medium at pH 8.0 and temperature 30ºC, pH 7.0 and temperature 350C, respectively.
Key words: Protease, pH, temperature, Aspergillus flavus and Aspergillus niger