Staphylococcus aureus is an adaptable, opportunistic pathogen with abilities to persist and multiply in a variety of environments and cause a wide spectrum of diseases in both humans and animals. Atotal of 144 samples from different sources (human,large animal,poultry and rabbit) were examined bacteriologically and biochemically. The results in this study revealed that out of total 97 isolates suspected to be Staphylococci, 42 isolates were positive for tube coagulase test (TCT) at a percentage of 43.2%. The antimicrobial sensitivity pattern of Staphylococcus aureus against 14 commertially available antibiotic discs showed 100% resistance to Kanamycin followed by Ampicillin (93.33%), Erythromycin (86.67%), Chloramphenicol (73.33%), Norfloxacin (73.33%), Cloxacillin (66.67%),Oxytetracycline (60.00%), Ciprofloxacin (60.00%), Sulphamethoxazol (46.67%), Gentamicin (40.00%), Amoxicillin (26.67%), Enrofloxacin (26.67%), Oxacillin (13.33%) and The least was observed with Cephalotin (6.67%). All 15 clinical isolates (4from human-6from large animal-3from poultry-2 from rabbit) tested by multiplex PCR were positive for clfB (100%). The other genes were heterogeneously detected:13 (86.66%) isolates(4human-5large animal-3poultry-1rabbit) of the strains were positive for clfA,7 (46.66%) isolates(3human-2large animal-1poultry-1rabbit) were positive for cna ,5 (33.33%) isolates(2human-2large animal-1poultry-0 rabbit) were positive for bbp.
Key words: staphylococcus aureus, isolation , PCR.