Objective: To evaluate the expression of p53 and Ki67 immunohistochemistry (IHC) as an adjunct to morphology in differentiating low and high-grade non-muscle invasive papillary urothelial carcinomas.
Methodology: This prospective cross-sectional study was carried out at Shifa International Hospital Islamabad and included 50 cases of papillary urothelial carcinoma assessed morphologically. IHC for p53 and Ki67 was performed on all cases and was analyzed using the Allred scoring system. Statistical analysis was done using Kendall's tau b test using SPSS 25.
Results: Among 50 cases, 64% were males and 36% females. 62% cases were high grade, and 38% of low grade papillary urothelial carcinoma. 50% of cases were staged pTa and 50% staged pT1. Total scores (TS) for p53 and Ki67 were high in high grade tumors while low in low grade tumors. Statistical analysis showed a significant association between proportionate score (p:0.01) and TS (p:0.01) indices for p53 and grade of tumor. Similarly, a significant association between proportionate score (PS) (p:0.01) and TS (p:0.01) for Ki67 with grade was also found.
Conclusion: Our study concludes that p53 and Ki67 IHC can be used as an adjunct to morphology in grading urothelial carcinomas.
Key words: Grade, Ki67, p53, papillary urothelial carcinoma.
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