Prasarinyadi Kashayam is used to treat Vata illnesses and joint discomfort in Ayurvedic system of medicine. It is mostly used to alleviate musculoskeletal issues and joint pain. It has traditionally been used to treat cervical spondylosis, frozen shoulder, headaches, and lower back pain and stiffness related to lumbar spondylosis. The present work was aimed to develop the pharmacognostic parameters for the standardization and quality evaluation of the Prasaranyadi Kashayam. In house polyherebal formulation was prepared as per the previously prescribed method and physichochemical parameters were evaluated as per standard protocol and showed loss on drying 99.31%, Total ash 4.911±0.120%, water soluble ash: 3.022, acid insoluble ash: 0.489. Total phenolic content was estimated with FC reagent and expressed as gallic acid equivalent (9.95 + 0.004 mg/g). High performance thin layer chromatography (HPTLC) fingerprint profiling was obtained, using Toluene: Ethyl Acetate: Formic acid (2.5: 2.0: 0.5) as mobile phase. The characterization parameters presented in this paper may serve as standard reference for quality control analysis of Prasaranyadi Kashayam.
Key words: Standardization, HPTLC, Finger printing, Prasaranyadi Kashayam
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