Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article

AJVS. 2015; 47(1): 166-174


Genetic Diversity of Mannheimiahaemolytica strains

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied.

Abstract
Mannheimiahaemolyticais the principal bacterial pathogen associated with bovine respiratory disease (BRD). As an opportunistic pathogen, M. haemolyticais also frequently isolated from the respiratory tract of healthy cattle. This study examined the incidence of M. haemolytica using deep nasal swabs from diseased cattle, buffaloes, sheep and goat as well as from their closely contact apparently normal animals (n = 225) and pneumonic lung tissues (n=90) then identification of the isolates by conventional culture method, biochemical and confimed by molecular tecqnique, finally assessment the genentic diversity between the confirmed isolates. Out of 315 samples examined, Mannheimia was isolated in 5% (4/75) ofcattlenasal and lung samples and 3.33%(3/90)of sheep samples. Identification of these isolates were confirmed by polymerase chain reaction (PCR) using species specific primer (PHSSA) derived from serotype specific antigen (ssa) gene. The amplification of genomic DNA obtained was corresponding to the anticipated size of 237bp.PCR was used to screen isolates for virulence outer membrane lipoprotein (gs60), O-sialoglycoproteinendopeptidase (gcp) and antimicrobial resistance (blaROB-1)genes. The virulence genes gs60 and gcp were present in all isolated M. haemolyticaat 329 and 227bp respectively, while antimicrobial resistant blaROB-1 gene was present in only two isolates at 685pb. Finally assessment the genetic differences among M. haemolytica isolates by partial genome sequencing of (gs60) gene blaROB-1 gene.

Key words: Mannheimiahaemolytica strains, bovine, respiratory, diseases



Similar Articles

Effects of selenium-containing compounds on Cu/Zn-induced neuronal cell death and potential application as therapeutic agents for neurological diseases.
Tanaka KI, Shimoda M, Kawahara M
Neural regeneration research. 2022; 17(2): 311-312

Striatal oxidative damages and neuroinflammation correlate with progression and survival of Lewy body and Alzheimer diseases.
Li H, Knight WC, Xu J
Neural regeneration research. 2022; 17(4): 867-874

Rates of Coinfection Between SARS-CoV-2 and Other Respiratory Viruses in Korea.
Kim YG, Park H, Kim SY, Hong KH, Kim MJ, Lee JS, Park SS, Seong MW
Annals of laboratory medicine. 2022; 42(1): 110-112

Clinical Evaluation of the Rapid STANDARD Q COVID-19 Ag Test for the Screening of Severe Acute Respiratory Syndrome Coronavirus 2.
Kim HW, Park M, Lee JH
Annals of laboratory medicine. 2022; 42(1): 100-104

Prevalence of a Single-Nucleotide Variant of SARS-CoV-2 in Korea and Its Impact on the Diagnostic Sensitivity of the Xpert Xpress SARS-CoV-2 Assay.
Hong KH, In JW, Lee J, Kim SY, Lee KA, Kim S, An Y, Lee D, Sung H, Kim JS, Lee H
Annals of laboratory medicine. 2022; 42(1): 96-99


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites