Pharmacognostic standardization and HPTLC fingerprinting analysis of Sahacharadi Kashayam: a classical ayurvedic polyherbal formulation
Paras Sharma, Prashant Kumar Dhakad.
Abstract
Multiherbal compositions are widely practiced in ayurvedic medical system, and kasayam (decoction) is one such dose. Sahacharadi Kashayam is a traditional ayurvedic polyherbal medicine recommended by ayurvedic doctors for chronic and acute back pains, lower limb disorders, and conditions that mainly affect the nutrient level in the blood and the bone. The aim of this study was to develop pharmacognostic parameters for the standardization and quality evaluation of the Sahacharadi Kashayam. In-house polyherbal formulation was prepared as per the previously prescribed method, and physicochemical parameters were evaluated as per standard protocol and showed loss on drying at 99.31%, total ash content at 4.911 ± 0.120%, water-soluble ash at 3.022, and acid-insoluble ash at 0.489. Total phenolic content was estimated with FC reagent and expressed as gallic acid equivalent (9.95 + 0.004 mg/g). High-performance thin-layer chromatography (HPTLC) fingerprint profiling was obtained, and the quantity of total tannins was estimated as tannic acid equivalent using HPLC (0.47%). The results obtained from the study could be utilized as a reference for the quality control and quality assurance of Sahacharadi Kashayam.
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