Herein we describe the preparation, characterization and utilization of of chitosan nano particles for the intracellular delivery of the poorly cell-penetrating antibiotic e.g. Ciprofloxacin, Chlortetracycline hydrochloride and Gentamycin sulfate to improve their treatment of bacterial infections. Chitosan nanoparticles were prepared via the ionic gelation of chitosan with tri polyphosphate anions. Several parameters were studied to obtimize the particle size of chitosan nanoparticles, here we select the concentration of chitosan and the concentrations of sodium tri poly phosphate (TPP) as obtimizing parameters and the other factors stay constant such as pH of solution and ultrasonication time. Chitosan nanoparticles formed characterized by using FT-IR and transmission electron microscope (TEM). Results show that chitosan nanoparticles and its loaded antibiotics kill and inhibits the growth of gram (+) and gram (-) bacteria tested due to nanoparticles structures, and the antibacterial activity increased with increasing the anti biotic content.
Key words: Chitosan nanoparticles, Ciprofloxacin, Chlortetracycline hydrochloride, Gentamycin sulfate Ionotropic gelation, Antibacterial activity and Drug Delivery
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