The normal mean serum activities of ALT, AST, ALP and GGT were 50.0 ± 3.53, 130.0 ± 7.29, 323.6 ± 32.09 and 10.11 ± 1.28 IU/L with 95% confidence interval of 42.02 to 57.98, 113.51 to 146.49, 251.0 to 396.19 and 7.15 to 13.07 IU/L, respectively. Alanine aminotransferases(ALT) and GGT was found to be sufficiently stable up to the study period of 14 days at both 4 ºC and -20 ºC whereas unstable at room temperature. Aspartate aminotransferase was more stable at 4 ºC up to 11 days and 8 days at room temperature and was stable only 2 days at room temperature. Alkaline phosphatase showed great variation upon storage as compared to other hepatobiliary enzymes and it is suggested that its estimation should be performed in fresh serum samples to get a more accurate result. Thus the present study reveals specific reference values for each serum hepatobiliary enzyme in buffaloes of hot humid tropics. From these results it is also advisable to consider stability of each serum hepatobiliary enzymes for different animals separately before preserving sera samples to get more valid and reliable result.
Key words: Physical Baseline Values, Days of Storage, Storage temperature, Murrah Buffaloes, Hepatobiliary Enzymes
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