The root of Sida acuta Burm. F. is useful in nervous and urinary diseases, disorders
of blood and bile and in chronic bowel complaints. Calcium oxalate is the most important
constituent of urinary stone crystals and several research workers have been attracted to carry out
in-vitro studies on urinary stone crystals. However, the inhibitory effect of the root of S. acuta
on calcium oxalate crystal growth in-vitro has not been carried out. In the present work an attempt
has been made to grow crystals of calcium oxalate and to study the inhibitory effect of the
methanolic and aqueous extracts of the root of S. aucta on calcium oxalate crystal growth invitro.
Calcium oxalate crystals were grown in silica gel media in Hanes tubes by single diffusion
method. The crystal growth after the addition of the methanolic and aqueous extracts in 20 mg/
5 ml and 10 mg/5 ml doses was studied. In both the extracts there were reductions in sizes of
calcium oxalate crystal columns and also the size of the individual calcium oxalate crystals when
compared to that of the controls. The results of in-vitro experimental models, IR and SEM together
attribute an inhibitory capacity with respect to calcium oxalate crystals for the methanolic and
aqueous extracts of Sida acuta.
Key words: Urinary disorders, Calcium oxalate, Crystal growth, Sida acuta
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