Abstract

Diabetes is a global disease problem that is thriving due to its synthetic medicine sustaining antibiotic resistance. Purslane leaves (Portulaca oleracea) with a high content of ω-3 fatty acid, particularly α-linolenic acid (ALA), have emerged as an alternative antidiabetic herbal medicine. This study aims to identify the potential of purslane leaf extract as an antidiabetic herbal medicine by analyzing the target protein tyrosine phosphatase 1B (PTP1B) to the ALA through molecular docking analysis. The research results showed that the highest extract volume yield was achieved in 120 minutes and a speed of 1,000 rpm using the stirring maceration method. The extract results with several solvents have properties that are appropriate to the standards. Sample E36 shows the most optimal potential because of the large number of ω-3 fatty acid functional groups then which will be analyzed further. The gas chromatography-flame ionization detection chromatogram shows a high ALA content in E36 and molecular docking analysis shows a binding affinity value of –3.4 kcal/mol to the target protein PTP1B, with hydrogen bonds and hydrophobic interactions. This compound has the potential for development as an anti-diabetic herbal medicine, with further exploration for efficacy in vivo and in vitro.

Key words: Antidiabetic, Purslane Leaves, ω-3 Fatty Acid, Stirring Maceration, Characterization, Herbal Medicine