Administration of cyclophosphamide to albino mice at a dose level of 7 mg / kg body weight, 3 times/ week for 8 weeks induced various histopathological changes in the liver such as hepatic tissue impairment, cytoplasmic vacuolization of the hepatocytes, congestion of blood vessels and leucocytic infiltrations. Moreover, the liver enzymes, ALT and AST, as well as glucose were increased in sera of treated mice. Histochemically, the hepatocytes showed increase in glycogen and decrease in total proteins, DNA and RNA. Treating animals with cyclophosphamide and aqueous extract of fenugreek seeds at a dose level of 0.4 mg/kg b. wt led to an improvement in both histopathological and histochemical pictures as well as biochemical parameters. These results proved that fenugreek had an ameliorative effect against liver injury induced by cyclophosphamide. This may be due to its antioxidant activity.
Key words: Cyclophosphamide, Mice, Fenugreek, Histology, Histochemistry, Biochemistry
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