The survival and infection rates of Bulinus truncatus exposed to Schistosoma haematobium or Echinoparyphium recurvatum were studied. The results showed that the infection rate of B. truncatus snails with E. recurvatum was higher than that by S. haematobium. Moreover, E recurvatum exerted a negative effect on the survival rate of snails. The results obtained also revealed that free amino acids showed variation in their concentrations and percentages of FAA in haemolymph and tissue of infected snails with two parasites as compared to control snails. Aspartic. Glutamic. Glycine, Threonine, Alanine, Methionine, Cystine, Tyrosine, Phenylalanine, Lysine and Luecine were increased in the tissue, but Serine, Histidine, Arginine, Proline, Valine and Isolucecine were decreased. In the haemolymph Histidine, Alanine and Proline were increased; on the other hand Aspartic, Methionine, Cystine, Tyrosine Phenylalanine and Lysine were decreased. Glutamic, Serine, Glycine, Arginine Threonine, Valine, Isolucecine and Luecine were deficient in the haemolymph of infected snails with S. haematobium and E. recurvatum. However, there are two characteristic bands (140.82 KDa & 14.42 KDa) appear in the electrophoritic pattarn of tissue protein B. truncatus snail infected with E. recurvatum at intervals of two and four weeks but there is only one specific band (100.9 KDa) characteristic for B. truncatus snails infected with S. haematobium.
Key words: Bulinus truncatus - Echinoparyphium recurvatum Free amino acids -protein- Schistosoma haematobium
|
