A seven month assessment study of microbiological profile of enteric pathogens in stool specimens of children aged under 5 years diseased with acute diarrhea and antibiotic sensitivity test of isolated bacterial agent were performed on a total of 250 diseased children case, from August 2009 to February 2010 in El-Thora hospital, El-Beida city, Eljabal El-Akhdar, Libya. All specimens were examined for various bacterial pathogens. Isolation rates in positive cases were: E. coli (55.3%), Salmonella spp. (32.5), and one isolate of Shigella sp. (0.8%). The isolation rate was highest in the summer, and was higher in males than in females. During the study the parasite Entamoeba histolytica represented (11.4%) of positive cases. The majority of the enteric pathogens which were detected were among children 24 months of age and less, but the highest percentages of bacterial infection and of sever dehydrated children were in age group equal to or less than 12 month. In this study severe dehydration was represented in (46%) of positive cases whereas moderate dehydration in (54%). Most positive cases were in bottle fed babies, the risk factor underscores need for mother education to the role of breast feeding in preventing diarrhea disease of microbiological origin. In this study all E. coli and Salmonella spp. isolated showed high resistance rate to Ampicillin, Trimethoprim–Sulphamethoxazole, and Carbenicillin. Both E. coli and Salmonella spp. showed comparable resistance to Tetracycline.
Key words: Children, Infectious diarrhea, E. coli, Salmonella spp.
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