This study was designed to evaluate the healing potential of rosemary (Rosmarinus officinalis) callus extracts on induced infected skin injuries in different experiments. Callus was induced on leaf explants and maintained for 30 days on Murashige and Skoog medium supplemented with 0.5 mg/l of benzyl adenine (BA) and 2.0 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D). Induced injuries in mice skin were made artificially by infection with the pathogenic bacterium Staphylococcus aureus. The healing potential of the extract was followed up and compared with those administrated the commercial skin ointment antibiotic Samacycline. Ethanol callus extract showed an antibacterial, anti-inflammatory and healing potential at a concentration of 80 mg/ml. Injuries were fully recovered after 12 days in animals treated with callus extracts while took 17 days in those treated with Samacycline. Incubation of Staphylococcus aureus with callus extract for 24 hrs and then applied on mice skin enhanced the recovery after 8 days only. The data clearly show that rosemary callus extracts are effective against mice injuries, with higher potential than Samacycline.
Key words: Antibacterial, Callus, Ethanol extract, Induced injury, Rosmarinus officinalis, Samacycline
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