Enrofloxacin (EFX) is an antibacterial drug associated with inhibition of many enzymes and many other effects. The aim of the present work is to investigate the protective effect of green tea extract (GTE) on EFX-induced kidneys abnormalities in albino rats. The rats were divided into five groups (AE); each group contains six rats. Group A was kept as control, whereas rats of group B were injected intraperitoneally with a daily dose (75 mg/kg body weight) of enrofloxacin for 10 days. In the remaining groups (CE), the animals were treated daily with one of the following three concentrations i.e. 1%, 1.5%, and 3% of freshly prepared green tea extract (GTE) daily the above mentioned dose of EFX for 10 days. At the end of the experiment, small pieces of kidney tissue were taken and processed for histological and histochemical examinations. The histological results revealed progresses nephrotoxic effects on the glomeruli and acute necrobiotic changes in both the cells of the proximal and distal convoluted tubules after treating the animals with EFX. Moreover, presence of hyaline casts in the tubules lumina with loss of the microvilli, disturbance of basal membranes enfolding and large vacuoles were recorded. The histochemical results showed depletion of the polysaccharides as well as the total proteins in glomeruli and lining epithelial cells. The wall of the tubules indicated marked decreases in many enzymatic activities. Besides, EFX-co administration of aqueous extract with GTE produces cytoprotective effects. In addition, marked improvements in the levels of glycogen and total proteins were recorded. Accordingly, it was concluded that aqueous extract of GTE improves the histological and the histochemical alterations induced by EFX at altitude of the different doses. More improvement was observed after the consumption of higher dose (3%).This may be attributed to the antioxidant properties of GTE constituents.
Key words: Green tea, Enrofloxacin, Rat, Kidneys, Histology, Histochemistry, Ultrastructure
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