The present study dealt with the molecular characterization of five digenean parasites collected from the intestines of different hosts in Egypt. The hosts collected from Dakahlyia Governorate were Bufo regularis, Bagrus bayad and the ruminants (cows and buffaloes), while those collected from Cairo and Giza were Bufo regularis and the rat Rattus rattus. The isolated parasites were classified belonging to digenetic trematodes and this was elucidated by molecular analysis of their DNA. Based on morphological characterization the isolated parasites were, Paramphistomum microbothrium and Carmyerius gregarious from the rumen and reticulum of cows and buffaloes; Mesocoelium monas from Bufo regularis; Acanthostomum absconditum and Haplorchoides cahirinus from the freshwater Bagrus bayad. Four general primers (P1, P2, P3, and P4) were used for RAPD-PCR to differentiate between these species. Also specific primers were used for the differentiation between the five digenean species examined. Three specific primers (ITS3/4, ITS4/2, and ITS5/4) were used. Each primer alone provides a good resolution to differentiate between the two studied Paramphistomid species, but they are not suitable to characterize the other three examined digenean ones.
Key words: Digenetic trematodes, Bufo regularis, Bagrus bayad, Cows, buffaloes, Primers: P1, P2 & P3, Egypt.
|
