The genetic fingerprinting in six accessions of barley (Hordeum vulgare L.) was investigated by rapid analysis. The accessions were collected from different regions of Saudi Arabia (Al-Taif, Hail, Al-Qassim, Asir, Al-Baha, and Jazan). A total of 91 genetic bands were detected using eight RAPD-PCR primers. The two primers OP-N1 and OP-H1 showed a higher ability to detect high rate of variation between the studied samples .The average variation rate obtained by the eight primers was 92.86%. Cluster analysis based on RAPD-PCR data analysis divided barley samples into two groups, The first group consist of accessions from Al-Baha and Jazan, which shown the highest degree of genetic convergence, and the second group consist of accessions from Asir and Al-Taif, which showed a close genetic relation, while the accessions from Al-Qassim and Hail associated with the second group by a percentage of 57% and 52%, respectively.
Key words: Barley, Finger print, PCR, Molecular markers
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