In the present study, microspore developmental stages of Solanum melongena L. Ishtar F1 hybrid were determined cytologically. The size and appearance of flower buds and anthers were determined morphologically. Cold pretreatment was applied to flower buds for 24, 48, or 96 hrs in order to induce the androgenetic ability. High percentage of callus induced on untreated anthers grown on Pelletier To medium which supplemented with 4 mg/l Naphthalene acetic acid (NAA) and 1 mg/l (kin). Different levels of kin were used for the induction of organogenesis in callus cultures obtained from anthers of control and cold pretreated flower buds. Callus tissues initiated roots when 0.1 mg/l kin was supplemented to the Pelletier To medium. Containing 5 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and of kin, C medium gave high percentages of callus (10%) and normal-looking embryos (3.3%) obtained on anthers incubated in continuous dark after transferring to R medium which supplemented with 0.1 mg/l kin.
Key words: Solanum melongena, anther culture, cold pretreatment, light or dark regimes
|
