Sesame (Sesamum indicum L.) is a vital oil seed crop highly rich in sesamin, sesamolin, and tocopherols, which are some of its nutrient and antioxidant components. Sesame is rich in genetic resources with vast germplasm, tolerance to various biotic and abiotic stresses, and quality-related traits. Therefore, a comprehensive study on searching important genes/quantitative trait loci (QTLs) is needed to get better oil from the plant. Pure DNA is the prerequisite to perform various molecular-level functions, including gene amplification, genomewide association studies, and other molecular breeding applications used in crop improvement. However, it also has high polysaccharides and secondary metabolites that interfere with DNA extraction, and precise quantification of DNA is essential for DNA-related biological functions. Several DNA extraction methods, such as cetyl-tetra methyl ammonium bromide (CTAB), sodium dodecyl sulphate (SDS), and commercially available kits, are present and used in molecular biology applications. Due to the complexity and interference of polysaccharides and polyphenols, the available methods required numerous modifications in the protocol to get pure and intact DNA. This study illustrates several modifications to get the pure DNA using the CTAB-based method with the additional step of adding a high salt concentration, which results in a better quantity and quality of DNA extracted from sesame leaves and root tissue. The lysis buffer and cold storage to efficiently eliminate the polysaccharide components were found to be limiting factors affecting DNA yield; therefore, buffer composition, along with storage duration, was modified in the present study. This method worked well to get good quality and quantity of DNA and will help researchers conduct next-generation sequencing and other genetic investigations directly associated with high-quality DNA in the oilseed crop.
Key words: DNA extraction, CTAB, oil seeds crop, polysaccharides, mucilage
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