Defects in the natural mechanisms of coagulation system resulting in thrombus formation in blood vessels are the third most common cause of death and disability globally. Anticoagulants and thrombolytics available are associated with different adverse effect and subsequent complications. This study attempts to evaluate the anticoagulation effect of the methanol extract of Manihot esculenta tubers (MEMET) using Albino rat models. Qualitative phytochemical screening and acute toxicity studies were conducted using standard procedures. The in vitro thrombolytic model was used to conduct assay on activated partial thromboplastin time (APTT), prothrombin time (PT) and Clot lysis (CL) while in the in vivo assay, bleeding time (BT) and clotting time (CT) were evaluated. Streptokinase, heparin and distilled water were used as controls, respectively. The phytochemical screening of the MEMET revealed the presences of carbohydrates, saponins, tannins, phenols, cardiac glycosides, triterpenoids and alkaloids however, flavonoids, diterpenes and proteins were found to be absent in this study. Oral acute toxicity study gave an LD50 value of >2000 mg/kg. The MEMET at the highest dose of 625mg/kg showed significant (p
Key words: Blood coagulation, Manihot esculenta, Cassava, Bleeding time, Clotting time
|
