In this study, we have tested alcoholic extracts (60%) from four Beninese plants: Ocimum gratissimum L., Acanthospermum hispidum DC, Caesalpinia bonduc (L) Roxb and Calotropis procera W. T. Aiton. They are used by the healers to prevent opportunistic diseases associated to HIV-AIDS; on six strains such as: Escherichia coli O 157H7, Staphylococcus aureus ATCC 25923 which resist to methicillin (MRSA), Salmonella typhi, Klebsiella pneumonia, Candida albicans ATCC 10231, and Mycobacterium bovis BCG 040812 which cause microbial infections associated with HIV-AIDS. The results show that all the extracts are bacteriostatic and fungistatic but only the hydro-ethanolic extracts of Acanthospermum hispidum (HE2) and of Caesalpinia bonduc (HE3) presented antibiotic power (respectively ap = 2 and ap = 4) on Candida albicans ATCC 10231. The Mycobacterium bovis BCG shown resistance to tested extracts (CMI > 250 µg/mL). The two fungicidal extracts HE2 and HE3 did not show harmful effects on the cells WI38 with an IC50 > 100 µg/mL for HE2 and IC50 = 50 µg/mL for HE3. The successive bio-guided purifications of extracts HE2 and HE3 permitted isolation of.three antibacterial compounds: Flavanone (M1); stigmasterol (M2); and quercetin (M3). The three isolated compounds possess antibiotic power (ap 31) on tested strains and are not toxic on shrimp larvae (LC50 0.30 0.17 mg/mL).
Key words: Microbial infections, HIV-AIDS, Beninese plants, cytotoxic activity, antimicrobial activity, chromatographic methods.
Key words: Key words: Microbial infections, HIV-AIDS, Beninese plants, cytotoxic activity, antimicrobial activity, chromatographic methods.
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