Ovarian torsion is a gynecological emergency that can lead to ovarian ischemia-reperfusion injury (IRI). This study aimed to investigate the potential protective effects of carvedilol against ovarian IRI in rats. A total of 24 female rats were divided into four groups: control, ischemia (I), ischemia-reperfusion (IR), and ischemia-reperfusion with carvedilol treatment (IR-C). Ovarian ischemia was induced by clamping the ovarian pedicle for 4 hours, followed by reperfusion for 4 hours. The IR-C group received carvedilol treatment 30 minutes before reperfusion. After the experiment, the ovaries were removed and evaluated for hemorrhage, edema, and immunopositivity of Caspase 3, apoptosis-inducing factor (AIF), Microtubule-associated protein 1 light chain 3b (LC3B), and Tumor necrosis factor-alpha (TNFa). In the staining performed with Caspase 3 and AIF, moderate immunopositivity was observed in the I and IR groups (p
Key words: Carvedilol, ovarian ischemia-reperfusion, rats, immunopositivity
|
