Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis
Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir.
Abstract
Pseudomonas aeruginosa is naturally resistant to wide spectrum of antibiotics in current use and infections by this bacterium have been found very refractory to chemotherapy so in this study we attempt to study the incidence and resistance pattern of pseudomonas aeruginosa in cattle. The present study was carried on 150 samples randomly collected from different farms at El-Behera and Alexandria goveronates from mastitic cows.
The samples were directly transferred to laboratory where they were prepared for bacteriological examination for isolation of pseudomonas aeruginosa , Determination of anti-bacterial sensitivity test and isolation of plasmids. Result of this study showed that 15 out of 150 samples (10%) were positive for pseudomonas aeruginosa
Result of this study showed that 15 isolates of pseudomonas aeruginosa were tested against 9 antibacterial agents. The organism was sensitive to ciprofloxacin, vancomycin.Fifteen pseudomonas aeruginosa isolates were positive for analysis by using polyacrylamide gel electrophoresis.The carried plasmids with molecular weight of 18Kbp.Ih could be concluded that Pseudomanas aerogenosa was resistant to the most antibiotics and carrying plasmid DNA. Moreover, antibiotic is important for the persistence of plasmid DNA.
Keywords : Phenotypic-genotypic-plasmids-pseudomonas
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