Research Article |
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Molecular detection of Babesia ovis and blood parameters’ investigation reveal haematological and biochemical alterations in babesiosis infected Lohi sheep in Multan, PakistanMuhammad Sajid, Syed Atif Hasan Naqvi, Muhammad Riaz, Ummad Ud Din Umar, Nasreen Nasreen, Adil Khan, Mourad Ben Said. Abstract | | | Cited by 0 Articles | Background:
Babesia infections in sheep can cause a wide range of clinical and laboratory presentations. Changes in Blood parameters are a meaningful manifestation of physiological and pathological changes in an organism.
Aim:
Therefore, the present study was conducted to analyze and compare haematological and biochemical parameters between blood profiles of Lohi sheep naturally infected and un-infected with Babesia ovis, the main causative agent of ovine babesiosis.
Methods:
Initially, blood and serum samples from 67 Lohi sheep were collected, DNA was extracted and babesial infection was detected through PCR. Overall infection rate of B. ovis was 37% (25/67). Sixteen infected (experiment group) and sixteen uninfected (control group) sheep that were apparently healthy with no history of previous treatment for Babesiosis, were selected for haemato-biochemical analysis. Blood samples were analyzed through an automatic CBC analyzer, while serum collected from gel-vacutainers was analyzed for blood urea, blood urea nitrogen (BUN), creatinine and total bilirubin. Each parameter was compared between infected and un-infected animals using a paired t-test in Minitab Express™ software for statistical analyses.
Results:
Erythron comparison showed a highly significant (P
Key words: Ovine babesiosis, Molecular detection, Blood parameters, Lohi sheep breed, Pakistan
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