Background:
Thymus vulgaris extracts can play a significant role as alternatives for antimicrobial agents against bovine staphylococcus mastitis.
Aim:
This research's goal was to evaluate the antibacterial properties of an extract from Thymus vulgaris as an alternative to antibiotics on bovine Staphylococcus mastitis. Also, to know the effect of the extraction methods (hot alcoholic, cold alcoholic and hot water extract) on its effectiveness.
Methods:
Two hundred ten of cow milk samples from different areas of Basrah province had been suffering subclinical mastitis reported by using California mastitis test (CMT). Staphylococcus species were identified by conventional microbiological technique, GP24 Kit, and nuc gene. Antimicrobial activity of various concentration of Thymus vulgaris extracted (75, 50, 25) mg /ml with different methods of extraction (hot alcoholic, cold alcoholic and hot water extract).
Results:
Out of 210 samples, 99(47.1%) were positive California mastitis test (CMT), the identification rate of Staphylococci spp. by conventional microbiological technique and GP24 kit were 78(78.8%). Out of 78 isolates of Staphylococcus spp 48(61.5%) were identified as S. aureus, by using both molecular technique by using PCR and miniaturize Kit GP24, and employing the miniature GP24, the remaining 30 (38.5%) were determined to be different species of Staphylococcus. Antibacterial activity of various concentrations of Thymus vulgaris extracted (75, 50, 25) mg /ml with different methods of extraction revealed that hot alcoholic extract (100%) was more effective than cold alcoholic extract (66.7%), whereas, there is no effect on the bacteria species with the hot water extract.
Conclusion:
Thymus vulgaris extracts can play a significant role as alternatives for antimicrobial agents against bovine staphylococcus mastitis.
Key words: California, Staphylococcus Species, subclinical mastitis, Thymus vulgaris
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