Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts
Theerayut Thongrin, Watcharapol Suyapoh, Woro Danur Wendo, Prasarn Tangkawattana, Peerapol Sukon, Kanin Salao, Sutas Suttiprapa, Prasert Saichua, Sirikachorn Tangkawatana.
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Individual host susceptibility is believed to be a risk factor in the interaction between the host and the parasite. Since studying time series in humans is limited, animal models are replaced.
This study aims to explore and compare the pattern of inflammatory cell types along the biliary tract and their association with proliferative lesions in the early development of CCA from susceptible and non-susceptible animal models.
Thirty male Syrian golden hamsters and 30 BALB/c mice, serving as the susceptible and non-susceptible animal models, were used in this comparative study. The animals were infected with 50 O. viverrini metacercariae via gastric intubation. At days 1, 2, 7, 14, 28, and 56 post-infection (p.i.), five animals were randomly selected from each group and humanely sacrificed. The hepatobiliary tissues were collected and processed for histopathological study. Histochemical and immunohistochemical staining were applied to differentiate the inflammatory cell types. Kruskal-Wallis and Mann-Whitney tests were applied to assess all semi-quantitative and quantitative variables. The correlation between each variable was also analyzed using Spearman rank at a p-value
Key words: Inflammatory cells, Biliary, Proliferation, Opisthorchis viverrini, Susceptible and non-susceptible host