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Research Article

Innovations Agric. 2023; 6(0): 01-05


Microscopical, histochemical and fluorescence evaluation of Tectona grandis L. leaves dye extract

Amutha Karunanithi, Sagaya Giri Robert.




Abstract

Teak leaves can also aid in the treatment of inflammations, leprosy, skin diseases, and indolent ulcers. Dyes are important in human life because they are both safe and environmentally friendly. The term macroscopic refers to substances that can be seen with the naked eye, whereas the term microscopic refers to substances that cannot be seen without the use of a magnifying device. It is possible to identify and localise biomolecules and organelles at the cellular level in various types of cells and tissues using histochemical techniques. Fluorescence is the emission of light by a substance that emits electromagnetic radiation. The results shows that the Tectona grandis leaves have dorsal and ventral surfaces that are 30-60cm long and 20-30cm wide. Teak leaf powder was treated with diluted ammonia and H2SO4 to produce a yellow colour, indicating the presence of flavonoids. A few drops of Fecl3 produced a black colour, indicating the presence of tannin. Blue, green or red coloration indicates the presence of polyphenol, while dinitrophenol hydrazine indicates the presence of terpenoids. Under both regular and UV light, the fluorescence behaviour of teak powder under various situations was investigated.

Key words: Tectona grandis, Natural Dye, Collection and Authentication, Macro & Microscopic estimation, Histochemical, Fluroscence study.






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