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Research Article

AJVS. 2019; 12(2): 38-45


Isolation and Diagnosis of Pathogenic Bacteria from the Bowels of Local Chicken and Identifying Some of the Virulence Factors

Nawal. A. Kh. AlUbaidy;and Sumaya. Y. A. AL-Dabbagh.




Abstract

This study was conducted to the isolation and identification of bacteria from chicken intestine and livers in Mosul city. A total of 35 samples from intestine and 35 liver samples from local chicken were collected during a period from September 2018 to March 2019, the bacteria were diagnosed according to morphological, cultural and biochemical characteristics. The results showed (100%) positive to bacterial isolation for each samples of intestine and liver, (12) types of bacteria from (71) isolates for intestine, while (10) types from (36) isolates for liver. E. coli formed the highest percentage of intestinal isolates (28.16%), while Corynebacterium spp formed the highest percentage in liver isolates (33. 33%).The bacterial types were isolated from intestine included: E. coli (28.16%) Corynebacterium spp (25.35%), Enterococcus faecalis (15.49%), Klebsiella pneumonia (8.45%), Bacillus spp (7.04%), Proteus spp (5.63%), Staphylococcus aureus (4.22%), Lactobacillus spp (2.81%), Arcanobacterium pyogenes (1.4%), Citrobacter spp (1.4%). The bacterial types were isolated from liver included: Corynebacterium spp (33.33%), E.coli ( 19.44%), Staphylococcus aureus (16.6%), Bacillus spp (11.11%), Enterococcus faecalis (5.55%), Klebsiella pneumonia (5.55%), Pseudomonas aeruginosa (2.77%), Listeria monocytogenes (2.77%), Arcanobacterium pyogenes (2.77%). The bacterial types isolated from both intestine and liver were, E.coli, Corynebacterium spp, Staphylococcus aureus, Bacillus spp, Enterococcus faecalis ,Klebsiella pneumonia, Proteus spp and Arcanobacterium pyogenes. The virulence factors tests were used for some liver isolates which included, protease, licethinase, lipase, urease, coagulase and haemolysin.

Key words: Isolation, Diagnosis, Bacteria, Chicken guts, Virulence factors






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