In this study, Pediococcus pentosaceus VTCC-B-601 was investigated and characterized for bacteriocin production. This antimicrobial activities was produced strongly at the late exponential phase (5x108 CFU/ml), corresponding to the activity of cephalosporin (13.3µg/ml) against Salmonella typhimurium ATCC 19430, Pseudomonas aeruginosa ATCC 27853, Staphylococus aureus ATCC 25923, and Micrococcus luteus ATCC 10240. The antimicrobial activities were determined due to a bacteriocin having 3.5 kDa in molecular weight. This bacteriocin was lysed by proteinase K and stable with high temperature, and precipitated by ammonium sulfate from 40%- 60%. This work supplied a Pediococcus pentosaceus bacteriocin identification which was useful in food preservation, clinical use, and agriculture.
Key words: antimicrobial activities, bacteriocin, Pediococcus pentosaceus VTCC-B-601, thermostability
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