Lysiphyllum strychnifolium (W. G. Craib) A. Schmitz is a plant with various medicinal properties, including antioxidant activity and detoxification. In this study, we initially screened the phytochemical compounds in methanolic (MeOH) extract by gas chromatography-mass spectrometry (GC-MS) technique and blasted them with NIST17 library. After screening, some bioactive compounds, such as 1,2-Benzenediol (3.68%); 1,2,3-Benzenetriol (12.16%); 1,3,5-Benzenetriol (24.30%); 13-Docosenamide, (Z)-(3.41%); and D-allose (24.46%) with reported antioxidant properties were identified; Then, the next analysis of phytochemicals; including catechin and quercetin in MeOH, ethanolic (EtOH), and aqueous extracts by the high performance liquid chromatography (HPLC). The results revealed that catechin contents in all extracts fell within the range of 3.46–9.55 mg/g DW. However, quercetin is figured out in all extracts except in the EtOH extract, which may be due to its low content and the limitation of detection. Subsequently, we tested the free radical scavenging activities of all extracts using the ABTS method. It is noteworthy that all extracts exhibited significant antioxidant activities, showing a logarithmic pattern when plotting the graph of decolorization versus concentrations, unlike the linear pattern observed with the standard Trolox. Our study confirms that L. strychnifolium extracts have excellent antioxidant activities and represents a potential candidate for alternative source of antioxidant agents in medicinal application.
Key words: Lysiphyllum strychnifolium, phytochemical, GC-MS, antioxidant, ABTS
|
