Background and Objectives: Long term use of Antibiotics, sedentary lifestyle, and change in the diet pattern is responsible for dysbiosis of microbiota. Dysbiosis of microbiota is the etiological factors of chronic diseases. Probiotics are live microorganisms, which show beneficial health effects on hosts once consumed in sufficient amounts may be the prophylaxis approach for chronic disease. LAB group can be isolated and characterized from traditional dairy sources and fermented product. This study aimed at isolating, identifying, and in vitro characterizing (morphology, catalase, oxidase test 16 S rRNA Sequencing and Phylogenetic Analysis) LAB strains from traditional Sauerkraut.
Materials and Methods: Isolated strains were identified by Gram staining, catalase assay, and molecular identification methods; 16S rDNA gene sequencing, phylogeny study, antipathogenic study and antibiotic susceptibility Assay.
Results: The experiments revealed that Pediococcus pentosaceus DSM 20336 (T) strains, which were isolated sauerkraut shows a desirable tolerance to low pH and high bile salts, favourable anti-pathogen activity, and acceptable antibiotic susceptibility; hence, they could be considered as novel probiotic candidates for preventing and treating the chronic disease
Key words: Dysbiosis, Microbiota, Sauerkraut, Pediococcus pentosanes, probiotic
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