Medicinal plants and their endophytes are one of the efficient producers of diverse secondary metabolites with therapeutic importance. In the present study, an endophytic fungus Talaromyces spp. isolated from Syzygium samarangense was subjected to whole-genome sequencing and Antibiotics and Secondary Metabolite Analysis Shell (AntiSMASH) annotation to identify biosynthetic gene clusters (BGCs) of secondary metabolites and their biosynthetic pathways. The Funannotate results revealed that the Talaromyces isolate has a total of 30.5Mb genome consisting of 372 contigs, 372 scaffolds and 47.64% GC content. In addition, 114 tRNA, 12722 functional mRNA, 12721 CDS transcripts, and 12721 protein-coding sequences were predicted and annotated using various BLAST databases. AntiSMASH revealed the presence of 76 BGCs, including 28 T1 Polyketide synthase (T1 PKS), 10 Nonribosomal peptide synthetases (NRPSs), 9 terpene, 1 Indole, 12 NRPs like, 4 T1 PKS and NRPs like, 2 T1 PKS Indole, 1 NRPs like terpene, 3 NRPs T1 PKS, 1 Indole NRPs, 2 betalactone, 1 phosphonate, 1 fungal-RiPP T1 PKS and 1 Other type. The analysis also predicted the occurrence of enzymes involved in the biosynthesis of some of the important secondary metabolites such as Pyranonigrin, Squalestatin -S, Azanigerone -A, Asperterpenoid -A, Napthopyrone, Clavaric acid, and Fusarin.
Key words: Endophytic fungi, Secondary Metabolites, AntiSMASH, Talaromyces, Biosynthetic gene clusters, Whole genome Sequence
|
