Necrotic enteritis and the subclinical form of Clostridium perfringens infection in poultry are caused by C. perfringens type A, producing the alpha toxin, and to a lesser extent type C, producing both alpha toxin and beta toxin C and have become serious threats to poultry health.This study was undertaken to determine the activity of 4 commonly available disinfectants against C. perfringens spores after 5,7.5,10,15,30 and 60 minutes of contact under dirty conditions, Of the 4 products tested, calcium hypochlorite 1% and Germicidan KOK 4% achieved the required reduction in microbial viability (≥103-fold) for relatively long contact times of 30 min, under dirty (3% yeast) conditions, One product (Biosentury 904, 2% conc) achieved the required reduction in microbial viability (≥103-fold) for contact times of 10 minutes, while Prophyl 75,1% concentration. achieved the required reduction in microbial viability (≥103-fold) for contact times of 5minutes.Upon addition of formic acid 2% and urea 1% the required reduction improved for (Biosentury 904, 2% conc) and Prophyl 75,1% concentration and achieved after 5 minutes and after 7.5 minutes for Germicidan KOK 4% while calcium hypochlorite was not improved by addition of formic acid 2% or urea 1%. Application of surface test using the four disinfectants was used alone and in combination with formic acid 2% or urea 1% showed nearly the same results obtained in the suspension test.
Key words: Sporicidal activity, disinfectants, C.perfringens, poultry broiler litter
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