Biodiesel production of oleaginous yeast isolated from the Mount Makiling Forest Reserve
Irene G. Pajares, Princess J. Requiso, Lorenzo M. Fabro Jr, Kristine Rose M. Ramos, Asuncion K. Raymundo.
Abstract
Microbial oils from oleaginous yeasts are potential alternatives for sustainable biodiesel production. The Mount Makiling Forest Reserve (MMFR) in the Philippines is a tropical rainforest with a megadiverse ecosystem but with limited information on its microbial diversity. This makes it an attractive source of novel and potentially biotechnologically valuable microorganisms. Twenty-two out of 258 yeasts isolated from the barks, roots, canopy leaves, and associated epiphytic plant material of various forest trees in the MMFR, were potentially oleaginous. Using a nitrogen-limited medium containing glucose as a carbon source, BUB8 and NFR6, isolated from the upper bark of a Bagtikan and the fern-root of the Narra tree, respectively, showed the highest biomass and lipid production levels. BUB8 accumulated a higher lipid content than NFR6 when grown in the same medium with glycerol as a carbon source. The fatty acid methyl ester profile of the transmethylated microbial oil produced by BUB8 showed that oleic acid is the dominant species (C18:1 >C16:0 >C18:2 >C18:0 >C14 = C16:1). Finally, phylogenetic analysis of the ITS rDNA region classified BUB8 as a member of the Rhodotorula genus.
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