PdEVs (Plant-derived Extracellular Vesicles) are nano-sized, membranous vesicles released by plant cells for defense mechanisms. They contain nucleic acids, proteins, lipids, and other secondary metabolites. In the current study, we characterized PdEVs derived from medicinal plants and identified the metabolites present in the PdEVs. We isolated PdEVs using the PROSPR method from the apoplastic fluid of four different medicinal plants: Azadirachta indica, Murraya koenigii, Ocimum tenuiflorum, and Coleus amboinicus. The isolated PdEVs were characterized morphologically by HR-TEM, and functional group characterization was performed using FTIR. Furthermore, metabolomic analysis was performed using GC-MS for the PdEVs from Azadirachta indica, Murraya koenigii, Ocimum tenuiflorum, and Coleus amboinicus. We found the identified metabolites to possess anti-cancer, anti-microbial, and anti-fungal activities, substantiating the potential use of PdEVs as a potential source for therapeutic applications.
Key words: Extracellular Vesicles, metabolomics, medicinal plants, GC-MS, FTIR.
|
