Glucose concentration affects recombinant interferon α-2b production in Escherichia coli using thermo-induction system
Mesalhy S. Mansey, Kamel A. Ghareeb, Abdel Naser Moghazy, Mahmoud M. Tawfick, Mohamed M. Fouda, Nagib A. El Marzugi, Nor Zalina Othman, Hesham A. El Enshasy.
In the present work, a recombinant Escherichia coli strain was used for the production of interferon α-2b in both shake flask and bioreactor. The first part of this research focused on the investigation of the effect of glucose concentration on the kinetics of cell growth, recombinant protein production and acetate formation. Glucose supplementation to culture medium enhanced cell growth when added in concentration between 0-20 g/L. Further increase in glucose level reduces biomass production and enhances acetate accumulation in culture. On the other hand, the results clearly demonstrated that maximal interferon production of 27.7 mg/L was achieved in culture supplemented with 20 g/L glucose. Further improvement in recombinant protein production process was also achieved by scaling up from shake flask to 16-L stirred tank bioreactor using pH-stat cultivation strategy. The maximal volumetric interferon production in bioreactor batch culture was 44.5. mg/L after only 6 hours.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
We use cookies and other tracking technologies to work properly, to analyze our website traffic, and to understand where our visitors are coming from. More InfoGot It!
