An attempt was made for the isolation of colicinogenic E. coli from cow and sheep dung samples. Total of 112 E. coli isolates were collected from sheep and cow dung samples in which 63 isolates are from cow dung and 49 isolates are from sheep dung samples. In the screening for colicin production, two E. coli isolates C22 and C51 from cow dung sample and one isolate S39 from sheep dung sample showed activity against the pathogenic strain E. coli O157:H7. Colicin production by all the three E. coli isolates was found to be enhanced by 0.25mg/l of mitomycin-c and three hours of incubation at 370C. Plasmid DNA was isolated from colicinogenic E. coli strains by alkali lysis method and their molecular weight was found to be 6.5 kb. The transformation experiment carried out with the standard strain E. coli DH5 confirmed that the plasmids were col-plasmids. The three colicinogenic E. coli strains isolated in this study will be a candidate to develop as probiotic for veterinary applications.
Key words: E. coli, colicin, transformation, E. coli O157:H7
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
We use cookies and other tracking technologies to work properly, to analyze our website traffic, and to understand where our visitors are coming from. More InfoGot It!
