The study evaluated the response of two generations of random and inbred Wistar albino rats to the ulcerogenic effects of indomethacin. A total of 144 rats (age: 12 to 14 weeks) belonging to two generations (G) and two breeding groups (36 rats/group) were used for the study. The animals in each group were assigned to three treatments: 10, 20, and 40 mg/kg of indomethacin per os and then fasted of food (48 h) and water (2 h) prior to treatment. The rats were sacrificed 2 h post treatment; the stomach and its content was harvested for evaluation of ulcer lesion and gastric acidity. Ulcer index (UI) and gastric acidity (GA) were compared between doses of indomethacin for breeding groups, within and between generations, and for sex of rats within breeding groups and generations. Results showed significant (p < 0.05) effect of dose on UI in G1 randombred rats, and GA in G1 and G2 inbred rats. Effect of sex was significant (p < 0.05) in G1 inbred rats. Rats belonging to G2 generation had higher UI but lower GA than G1 rats. It was concluded that inbreeding enhances the sensitivity of albino rats to toxic effects of indomethacin. Thus inbred rather than randombred rats could be better materials in assessing the toxicity of pharmaceuticals, and chemical agents.
Key words: inbred rats, randombred rats, indomethacin, toxicity tests, ulcer index
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