Genetic diversity plays an important function in the improvement of germplasm which has a direct association with the crop productivity. A number of statistical methods have been employed to investigate genetic diversity among the genotypes of various crops. Approaches like principal component and cluster analysis are useful and most frequently used for identifying plant characters individually and assisting breeders in genetically enhancing attributes in wheat genotypes. This research was carried out at the experimental field of On-farm Research Division (OFRD), Bangladesh Agricultural Research Institute (BARI), Shyampur, Rajshahi, Bangladesh, to study the genetic diversity and selection of high yielding wheat genotypes with their important agronomic and physiological traits among studied genotypes in drought condition by using principal component and cluster analyses. A total of 70 bread wheat genotypes were evaluated in 7 × 10 alpha lattice design in non-irrigated drought conditions during 2018-2019 cropping season. The first four principal components (PCs) with eigen values greater than 1.0 accounting for 82.81% of the total observed variation among genotypes. Traits with maximum values in PC1 were spikes m-2 (SPM), thousand grain weight (TGW), ground coverage (GC), normalized difference vegetation index (NDVI), grain yield (GY), biomass (BM), and harvest index (HI) while PC2 comprised heading days (HD) and BM. The major contributors to PC3 were grains spike-1 (GPS) and GC, whereas the maximum value of trait in PC4 was in relative leaf water content (RWC). The principal component biplot selected 21 high yielding genotypes than the average yield as they were distributed on the positive side of the PC1. The cluster analysis grouped 70 genotypes into six diverse clusters. Cluster Ⅱ containing same 21 genotypes previously selected by principal component biplot provided the highest SPM (257.4), GPS (42.2), TGW (40.51 g), GC (0.27), NDVI(0.73), SPAD (44.24), RWC (88.33%), grain yield (3216kg ha-1), BM (8535 Kg ha-1) and HI (0.37) belonging to the lowest canopy temperature at vegetative stage (16.14˚C) and canopy temperature at grain filling stage (24.64˚C) and moderate HD (71.65 days). Based on the results of the current study the best genotypes can be used as important breeding materials in upcoming breeding schemes for drought tolerance.
Key words: Keywords: Wheat, cluster analysis, principal component analysis, canopy temperature, eigenvalue, Grain yield
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