Bovine viral diarrhea (BVD) is an economically significant diseases in the bovine industry causing severe economic losses and there is no specific treatment so the primary aim of control to detect and remove persistently infected cattle (PI carriers) which serve as a virus source by shedding the virus life along and responsible for the presence of the virus within herds. So early diagnosis of PI animal is the critical point. To our knowledge, there have been no reports about the disease’s situation in New valley province, Egypt. The goal of this study was to serodiagnosis of BVD infection by Serum Neutralization Test (SNT) and early diagnosis of PI animals using BVDV Ag (POC)Test and Double antibody Sandwich ELISA (DS. ELISA) .In this study a total of 320 cattle of different ages, sexes, and breeds (not previously vaccinated with BVD vaccines) were screened clinically for symptoms of BVD infection, 92 cattle out of the totally screened 320 cattle showed clinical signs arousing suspicion of BVD( respiratory manifestation, diarrhea, growth retardation, oral lesion, and abortion) and undergo SNT and the result shows the percent of infection was 31.5% (29/ 92) .20 animal out of 92 clinically diseased undergo BVDV Ag Point-of-Care Test for detection of PI animal, 11 out of 20 examined cattle (55 %) were positive. PI animal was found to be high in cattle aged>2 months – 6 months than in other age groups. Also, male cattle had a greater infection rate than female ones, and infection was high in Frisian than in native bread. For more accurate confirmation five positive PI animal undergo (DS. ELISA). All Five positive PI animal were positive in ELISA (100% compatibility).
Key words: BVD, ELISA, PCR, Epidemiology
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