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Assessment of the characterization antimicrobial and green synthesis of magnesium oxide nanoparticles using Psydium guajava - in-vitro analysi

S.B. Rajalakshmi, R Subhasree*, Rajalakshmanan Easwaramoorthy,Thiyaneswaran Nesappan.




Abstract

Aim: To assess the characterization, antimicrobial activity of green synthesised magnesium oxide nanoparticles using
Psydium guajava leaves.
Materials And Methods: Psydium guajava leaves were dried and ground, of which 2 g of the powder was used for the
study.the powder was then dissolved in 100ml of distilled water. 1 mg of magnesium chloride salt was dissolved in 20 ml
of distilled water. Both the solutions were mixed and subjected to centrifuge. After the preparation of the nanoparticle,
it was subjected for further investigation like SEM analysis, EDAX, UV-VIs, cytotoxicity and anti microbial tests. The
cytotoxicity was investigated using a brine shrimps bioassay.The number of surviving naupli cells were manually counted
under dissection microscope. The antibacterial activity was examined against four bacterial strains- E.coli,
E.fecalis,S.aureus,P.aurogenosa by agar well diffusion method .The inoculated plates were incubated overnight (for about
24 hours at 37 degree celsius) and the inhibition zone around the nanoparticle solution was calculated. The antimicrobial
tests were carried against standard antibiotic and the results were tabulated.
Results: SEM analysis showed a particle size of 70 nm and the UV -Visible spectroscopy showed a peak at 320nm confirming
the presence of magnesium oxide nanoparticles that is prepared using Psydium guajava. Cytotoxicty tests revealed that
83% of the brine shrimp cells survived .The anti microbial tests revealed that the nanoparticle was effective against two
micro-organisms -S.aureus and E.fecalis and their zone of inhibition was nearly equal to that of standard antibiotic against
which the organisms were incubated.
Conclusion: Psydium guajava synthesized magnesium nanoparticles were found to have good cytotoxicity and acceptable
anti-microbial activity especially against two most commonly harbouring micro-organism(S.aureus and E.faecalis) .Hence
they can be extended for use in soft tissue healing.

Key words: green synthesis, nanoparticles, antimicrobial, vitro analysis






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