Abstract

Background: Cyanthillium cinereum known as Poovamkurunilla in Tamil is one among the traditional medicinal plants
which has proven properties.
Aim: Hence, this study is aimed to evaluate the antimicrobial and anti-inflammatory properties of Cyanthillium cinereum
in two groups.
Methodology: The oral pathogens such as Streptococcus mutans, Staphylococcus aureus and Enterococcus faecalis and C.
albicans were used to assess the antimicrobial efficacy of Cyanthillium cinereum in two groups(Group 1 - Aqueous extract
of C.Cinereum leaves; Group 2 – C.Cinereum whole plant using agar well diffusion method at various concentrations that
ranges from 25 µL, 50 µL and 100 µL. Anti-inflammatory activity was evaluated using protein denaturation assay. Statistical
analysis was performed using SPSS version 23. Independent t test and One-way ANOVA were used for measure between
group analysis and Repeated measures ANOVA were used for measure within group analysis.
Results: Increasing the concentration increases the response too against oral microbes. Group 1 shows better antimicrobial
properties than group 2. Statistically significant differences were found on all concentrations taken into consideration.
Whereas both the groups show equal anti-inflammatory activity when compared with diclofenac. Statistically significant
difference was found on all comparisons except Group 1 versus Group 2 in all the concentrations (p=0.358).
Conclusion: The study revealed that C.Cinereum leaves exhibit strong antimicrobial activity against oral microbes except
Candida albicans. Both Group 1 and Group 2 exhibit better anti-inflammatory activity than standard values. Further it has
to be tested for its cytotoxicity and antioxidant activity for future usage of Cyanthillium cinereum in applications in the
pharmaceutical industry.

Key words: Microbial, Herbal dentistry,Anti-inflammatory, Aqueous extract