Pandanus amaryllifolius Roxb. (Pandanaceae) prop root was investigated for biological activities, i.e. antioxidant (DPPH radical scavenging assay) and antibacterial activity against Staphylococcus aureus DMST 2933 and Escherichia coli DMST 4212. The results showed that a crude extract of pandan prop roots exhibited antioxidant activity with IC50 of 230.24 ± 10.69 µg/ml, and it had a total phenolic content of 24.75 ± 0.74 mg GAE/g of TPC content and inhibited the growth of S. aureus DMST 2933 with 9.75 ± 0.35 mm of inhibition zone diameter. The prop root powder was used to develop a novel bacterial cellulose (BC) production using enzymatic hydrolysis. The maximum total soluble solids content at 2.67 ± 0.29 Brix was found when using prop root powder at 100 g/l with 4.0% (v/v) of the commercial enzyme (iKnowZyme® cellulase) after incubated at 50°C, pH 5.0 for 24 h. The hydrolysis pandan prop root was fermented at room temperature for nine days with Komagataeibacter xylinus AGR 60, yielded 13.5 ± 0.50 mm of thickness with 7.90 ± 0.10 g of dry weight. Scanning electron microscope and Fouriertransform infrared spectroscopy were used to characterize the physical and chemical structure of the BC produced from pandan prop root, revealing that pandan prop root has the potential for a novel BC production with bioactivities of antioxidant and antibacterial properties.
Key words: Pandan prop root, Bacterial cellulose, Enzymatic hydrolysis
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