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Original Article



Profiling of seminal plasma proteins to identify the fertility of Simmental bull with low semen quality

Abdullah Baharun, Annisa Rahmi, Dede Kardaya, Syahruddin Said, Mokhamad Fahrudin, Raden Iis Arifiantini, Ni Wayan Kurniani Karja.




Abstract

Objective: The present study analyzed the seminal plasma proteome and possible relationships between proteins and semen quality in azoospermic and normal Simmental bulls.
Materials and Methods: Fresh semen plasma samples from the Lembang Artificial Insemination Center were used for this study, including one bull (76´ ejaculate) with very poor semen quality/ azoospermia (poor fresh semen/infertile; PFS) and three bulls with normal semen quality (normal fresh semen; NFS) for proteomic analysis using a pooled system (NFS-Stud) (60´ ejaculate). The only males obtained with very low quality or azoospermia (PFS) had sperm motility of

Key words: Fresh semen; proteome; Simmental bull proteomic; seminal plasma






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