Cervical cancer is caused by persistent human papilloma virus types 16 and 18 infections. This cancer is classified as a malignancy due to its capability to invade and seed at distant sites to form metastases. Lymph nodes metastasis areis an important factor relating to cervical cancer survival. Our previous study reported eugenol from clove oil (Syzygium aromaticum) has anti-cancer effects by inducing apoptosis through p53 protein degradation inhibition in HeLa cancer cells. In this study, we continue to examine the anti-cancer eugenol effects as an anti-metastatic agent. A true experimental research with post-test only controlled group design was carried out on four groups of HeLa cell cultures receiving different eugenol concentrations. To identify the molecular mechanisms involved in the eugenol anti-metastatic effect, eugenol- treated HeLa cells were subjected to scratch wound healing and immunofluorescence staining assays. Both qualitative and quantitative approaches were carried out to analyze the obtained data. Quantitative methods such as one-way analysis of variance, Tukey’s multiple comparison test, and Pearson’s correlation were carried out. We show that eugenol effectively inhibits HeLa cell wound healing migration. Furthermore, we show the eugenol effect downregulated Snail-1 and vimentin protein expression and upregulated E-cadherin protein expression. These proteins are important regulators of epithelial-mesenchymal transition pathways.
Cervical cancer, E-cadherin, epithelial-mesenchymal transition, eugenol, HeLa cell,vimentin
Disparities in Genetic Testing for Heritable Solid-Tumor Malignancies.
Dillon J, Ademuyiwa FO, Barrett M, Moss HA, Wignall E, Menendez C, Hughes KS, Plichta JK
Surgical oncology clinics of North America. 2022; 31(1): 109-126
Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission.
KselĂkovĂˇ V, Zachleder V, BiĹˇovĂˇ K
Methods in molecular biology (Clifton, N.J.). 2022; 2382(): 89-101
Photoacoustic image-guided corpus cavernosum intratunical injection of adipose stem cell-derived exosomes loaded polydopamine thermosensitive hydrogel for erectile dysfunction treatment.
Liang L, Shen Y, Dong Z, Gu X
Bioactive materials. 2022; 9(): 147-156
Financial Toxicity and Shared Decision Making in Oncology.
Surgical oncology clinics of North America. 2022; 31(1): 1-7
Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue.
Curtis MA, Vedam-Mai V
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 201-208