As one of the most precious medicinal mushrooms, Trametes versicolor is widely used in the treatment and prevention of cancer. In an attempt to identify nutritional requirements, this study explores the influence of carbon source (C), nitrogen source (N), and cultivation substrate on the mycelial growth and yield of T. versicolor. The optimal C and N sources and their ratios for the mycelial growth of T. versicolor were fructose and yeast extract at a 3:1, respectively. T. versicolor cultivated on a substrate mixture of 62% sawdust + 30% rice husk + 3% wheat bran + 1% CaCO3 exhibited the highest biological efficiency (12.58%). The findings in this study will provide important information regarding spawn production and T. versicolor cultivation at an industrial-scale.
Key words: Trametes versicolor, nutritional requirement, mycelial growth, cultivation substrate
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