Objective: The objective of this study was to investigate the hypoglycemic activity of Wrightia tinctoria (Roxb) (R. Br. Linn., Family- Apocynaceae) total flavonoid (WTTF) fraction on alloxan induced hyperglycemia. Materials and Methods: Total flavonoid fraction from WTTF seed was separated and LD 50 calculated as 1006.93 mg/kg, i.p., following Organization for Economic Co-operation and Development guideline 423. The effect of total flavonoid fraction was observed in three different dose levels 50, 75, and 100 mg/kg. Hypoglycemic activity was carried out using alloxan induced hyperglycemia model. The parameters observed were body weight, blood glucose, total cholesterol, triglycerides (TG), low density lipoprotein, high density lipoprotein, urea and creatinine along with relative organ weight of liver, kidney, and pancreas. Results: Total flavonoid fraction of W. tinctoria did not have any significant effect on reducing glucose level. WTTF had significantly reduced creatinine (P < 0.001), TG (P < 0.05) level and relative weight of liver in diabetic rat at the dose of 100 mg/kg. Conclusion: All the findings indicate absence of antidiabetic activity in W. tinctoria seed but possible presence of liver and kidney protective property.
Key words: Alloxan monohydrate, flavonoid, seed, Wrightia tinctoria