Plant extracts and derived products are in recent times being investigated as potential sources of compounds with anticoccidial activity. In this study, an in vitro sporulation inhibition assay was carried out against the oocysts of Eimeria tenella in a laboratory setting. Freshly harvested oocysts of Eimeria tenella were exposed to different concentrations (50, 25, 12.5, 6.25 mg/mL) of Neemazal® and assessed for oocyst sporulation inhibition on 3, 5, and 7 days of incubation in microtitre plates. At 7 days post incubation, all the tested concentrations evaluated showed a sporulation inhibition rate above 90%. The result reveals that Neemazal® can strongly inhibit oocyst sporulation of E. tenella and can be employed in the control of avian coccidiosis.
Key words: neemazal, in vitro, sporulation inhibition, Eimeria tenella, chicken
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