Abstract

This research studied the synthesis of chitosan (CS) membrane with the basic component of polyphenol oxidase (PPO) from purple eggplant extract added with Cu2+ ions. The activity of the immobilized PPO in the CS- membrane was determined by the UV-visible spectrophotometer methods. The results showed that the membrane characteristics of CS-/PPO-Cu2+ purple eggplant extract: (diameter = 9 cm, thickness = 0.021 cm; PPO activity= 2,894.99 U). The performance of the membrane is designed as filtration with a dead-end system, with three times the filtration process, and ten times the use of a batch system membrane. The application of the CS-crude PPO- Cu2+ membrane was carried out on samples of artificial waste and textile waste solutions. The results achieved from the samples of artificial waste and textile waste, respectively, (the rejection = 68.77% and 63.69%; the conversion of phenol into catechol = 16.92% and 17.19% ; the conversion of phenol to quinone = 42.96% and 42.64%; flow rate = ±11 L/h and ± 5 L/h; membrane flux= 10,007.99 L/m2.h at a pressure of 1 bar; permeability= 300–1,000 L/m2.h.bar). The membrane lifetime is stable up to 8 times use from 10 times use

Key words: chitosan, membrane, polyphenol oxidase, purple eggplant extract