Abstract

Objective: The aim of the present study was reported to evaluate the dispersal pattern of T-bet stained intraepithelial lymphocytes (IELs) in the patients with the celiac diseases (CD).
Methods: 250 patients had participated in this study. These patients were diagnosed with the CD by relying on the assimilation of clinical pathology features. A routine process of duodenal biopsied tissues were conducted onto the formalin-fixed paraffin-embedded (FFPE) blocks. Hematoxylin and eosin (H and E), and T-bet were used to prepare and stain the sections. The distribution of intraepithelial lymphocytes was evaluated. IBM Statistical Package for the Social Sciences (SPSS) analytic software was used to analyze the results.
Results: T-bet stained cells counts and the rise of intraepithelial lymphocytes P-value = 0.001 are significantly correlated. The IELs distribution was regular distribution in the majority of cases and only one case had IELs distribution in the body of the villi. The IELs distribution pattern in CD cases might be an early indicator of the disease.
Conclusions: This can be used in the Diagnosing and follow up of the patients with celiac disease.

Key words: Celiac Disease, Intraepithelial Lymphocytes, Hematoxylin, Eosin, T-bet, Coeliac disease, Diagnosing, Gluten sensitive.